GERARDO MAZZELLA

 GERARDO MAZZELLA

Chapter  1
26 Pages

Peripheral Blood and Bone Marrow

WithFermina Maria Mazzella, Gerardo Perrotta

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A properly prepared and stained peripheral blood smear remains an essential component in the practice of laboratory hematology. Although many automated hematology instruments have lessened the need for peripheral blood smear review, none has been able to totally replace human evaluation. Blood from a fingerstick is preferable, although ethylenediaminetetraacetic acid -anticoagulated blood is used more frequently. The major disadvantage of a glass slide is the irregular distribution of cells, which may cause difficulty in evaluating platelets and leukocytes. An acceptable blood smear will cover one-half to two-thirds of the glass slide, with the feathered end producing a rainbowlike effect when held to the light. The erythrocytes are macrocytic, normocytic, or microcytic when compared to the nucleus of the mature lymphocyte. The platelets should be evenly distributed on the slide when making an evaluation. Platelets cluster on slides prepared from fingersticks and render estimates difficult.

Gerardo with her friend Gerardo

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